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ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Topic 1: Microbiology15-20%- Specimen collection and culture techniques
- Identification of pathogenic microorganisms
- Mycology, parasitology, and virology
- Bacteriology and antimicrobial susceptibility
Topic 2: Immunology and Serology5-10%- Immunologic testing methodologies
- Immune system principles and components
- Infectious disease serology
- Autoimmune and immunodeficiency disorders
Topic 3: Urinalysis and Other Body Fluids5-10%- Cerebrospinal, serous, and synovial fluids
- Urine formation and composition
- Semen and amniotic fluid testing
- Physical, chemical, and microscopic analysis
Topic 4: Hematology20-25%- Red blood cell physiology and disorders
- White blood cell morphology and diseases
- Routine and automated hematology procedures
- Hemostasis and coagulation testing
Topic 5: Blood Banking (Immunohematology)15-20%- Compatibility testing
- Transfusion reactions and adverse events
- Blood components and storage
- Antibody detection and identification
- Blood group systems and antigens
Topic 6: Clinical Chemistry20-25%- Electrolytes, acid-base balance, and blood gases
- Toxicology and liver/kidney function tests
- Carbohydrates, lipids, proteins, and enzymes
- Endocrinology and therapeutic drug monitoring
Topic 7: Laboratory Operations5-10%- Quality control and quality assurance
- Laboratory safety and regulatory compliance
- Laboratory mathematics and instrumentation
- Pre-analytical, analytical, and post-analytical processes

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Match the viruses below with their associated conditions.
1. Herpesvirus
2. Papovavirus
3. Rhinovirus
4. Rotavirus

A) Warts
B) Herpes
C) Common Cold
D) Gastroenteritis in infants


2. All of the following activities are associated with platelets EXCEPT:

A) Lysis
B) Aggregation
C) Adhesion
D) Release of granules


3. The combination of complement C4, C2 and C3 that binds to the antigen-antibody complex in the initial reaction step in the classical pathway of complement activation is called the activation unit.
Which of the following is the "activation unit" in the classical complement pathway?

A) C1, C2, C9
B) C5, C6, C7, C8, C9
C) C1
D) C4, C2, C3
E) C2


4. Provide the equivalent measurement for one gallon.
Question options:

A) 1 liter
B) 3.79 liters
C) 4.2 liters
D) 2.5 liters


5. Microcontainer collection order of draw differs from regular peripheral blood collection order of draw. When performing a capillary draw, the lavender top container is obtained first. This decreases the possibility of clots in the container which would invalidate the results of the complete blood count test.
After the lavender is drawn, other containers with anticoagulants would be collected, and containers without anticoagulants would be collected last.
A phlebotomist must perform a skin puncture to obtain capillary specimens for a complete blood count (CBC), a plasma-based chemistry test, and a serum-based immunology test. The microcollection containers that will be used are lavender top containing EDTA anticoagulant, green top containing heparin anticoagulant, and gold top containing no anticoagulant. Which microcollection container should be collected first?

A) Lavender top
B) Green top
C) Gold top
D) The order of draw is not important.


Solutions:

Question # 1
Answer: A,B,C,D
Question # 2
Answer: A
Question # 3
Answer: D
Question # 4
Answer: B
Question # 5
Answer: A

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